green monkey cells cos  (ATCC)

Journal: Light, Science & Applications

Article Title: Interferometric Image Scanning Microscopy for label-free imaging at 120 nm lateral resolution inside live cells

doi: 10.1038/s41377-026-02210-y

Figure Lengend Snippet: a Overview of a COS-7 cell. Scale bar 10 μ m. Arrows mark individual organelles that are visible in this particular optical section. Nucleus (N), mitochondria (M), actin cytoskeleton (A), plasma membrane and lamellipodum (L), the endoplasmic reticulum (ER) as well as vesicles (V). Notably, positive and negative contrast of the same type of organelle indicate slightly different axial positions with respect to the optical section. b Upper close-up from a showing ER, vesicles and lamellipodium. Scale bar 2 μ m. c Lower close-up from a showing ER and lamellipodium. Scale bar 2 μ m. d Confocal iSCAT evaluated for the close-up of g as indicated with white dashed square. (Left) Closed pinhole and (right) open pinhole analysis. e iISM-APR of the same region as d . Scale bars d , e 200 nm. f Line profiles of interference contrast of vesicle cross-section as indicated in d , e for closed pinhole (green), open pinhole (blue) and iISM-APR (red). g iISM-APR timelapse of close-up in c after additional background correction to increase visibility of organelles on flat-fielded background (see Methods). h Timelapse of close-up in g with exemplary four consecutive time points ( t 0 − t 4 ) at time increments of about 8.2 s. Upper red arrows indicate vesicle motion, lower red arrows indicate ER remodeling. Scale bars g , h 1 μ m

Article Snippet: African green monkey kidney COS-7 cells (ATCC CCL-70) were cultivated in DMEM supplemented with 10% heat-inactivated FBS.

Techniques: Clinical Proteomics, Membrane

Journal: bioRxiv

Article Title: Chromogranin A regulates the dynamics of neurosecretion through its interaction with phosphatidic acid

doi: 10.64898/2026.01.29.699889

Figure Lengend Snippet: (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 in COS-7 cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.

Article Snippet: African green monkey kidney fibroblast-derived COS-7 cells (American Type Culture Collection; CRL 1651) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Thermo FisherScientific) supplemented with 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin (Gibco, Thermo FisherScientific).

Techniques: Over Expression, Incubation, Fluorescence, Clinical Proteomics, Membrane

Journal: bioRxiv

Article Title: Chromogranin A regulates the dynamics of neurosecretion through its interaction with phosphatidic acid

doi: 10.64898/2026.01.29.699889

Figure Lengend Snippet: (a) TIRF-M imaging of COS-7 cells overexpressing and secreting CgA- and CgAΔPABD-pHluorin after 2 mM BaCl 2 stimulation. SG leading to exocytic events on whole cells are surrounded and indicated by yellow arrows. Scale bars: 10 µm. Below are represented typical images of CgA- or CgAΔPABD-pHluorin exocytosis events. (b) Quantification of the number of exocytosis events in COS-7 cells overexpressing CgA-pHluorin and CgAΔPABD-pHluorin after their stimulation. (n=4 independent experiments; 8 cells in CgA condition and 7 cells in CgAΔPABD condition). Data are represented as mean ± SEM. Each point is one analyzed cell and each color is an independent experiment. **p < 0.01, Mann-Whitney test. (c) Typical images of CgA-EGFP or CgAΔPABD-EGFP exocytosis events. Scale bar: 500 nm. (d) Curves represent the normalized variation of the fluorescence intensity of single exocytic events during 10 s from CgA-or CgAΔPABD-EGFP overexpressing COS-7 cells after 2 mM BaCl 2 stimulation. The mean ± SEM of 75 exocytosis of 5 different CgA-EGFP overexpressing cells in 3 independent experiments and the mean of 65 exocytic events of 7 different CgAΔPABD-EGFP overexpressing cells in 3 independent experiments. Data have been normalized between 0 (before exocytosis) and 1 (maximum intensity). (e) Plot representing the mean ± SEM of area under curves from CgA-EGFP or CgAΔPABD-EGFP secretion kinetic. Each point represents one exocytosis event, and one color represents one analyzed cell. ****p < 0.0001, Mann-Whitney test.

Article Snippet: African green monkey kidney fibroblast-derived COS-7 cells (American Type Culture Collection; CRL 1651) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Thermo FisherScientific) supplemented with 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin (Gibco, Thermo FisherScientific).

Techniques: Imaging, MANN-WHITNEY, Fluorescence